Protocols

Before starting your Proteomics experiment, please read carefully these guidelines.

Basic guidelines – UH Proteomics Core

– Sample preparation for protein identification from a gel band or spot

– Potential interference and contaminant ions in MS

Technical protocols:

– In-gel digestion protocol for MALDI MS

– In-gel digestion for protein identification – LC-MS with maXis and amaZon

– In-solution trypsin digestion

– Nano-LC with ion-trap MS analysis

– Peptide mass fingerprint by MALDI workflow

– MALDI Imaging

– MALDI Biotyper

– Data Analysis – maXis Impact

– Malaria Metabolomics Workflow

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